Laixin wang vice president of bioanalytical at novabioassays and. Pdf this article summarizes bioanalytical avenues for the determination of. In addition to the low assay specificity, the reported limit of the quantification of a competitive hybridization assay is 900 pm 5 ngml in plasma for a 15mer phosphodiester oligonucleotide 6. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Advances in quantitative bioanalysis of oligonucleotide. Importance of probe design for bioanalysis of oligonucleotides using. Pdf quantitatitive analysis of phosphorothioate oligonucleotide in. A ntisense oligonucleotides, sirnas, mirnas, aptamers, and other small. Oligonucleotides and nucleic acids interest group workshop. Quantitative bioanalysis of oligonucleotides laixin wang, ph. If you are a society or association member and require assistance with obtaining online access instructions please contact our. Quantitatitive analysis of phosphorothioate oligonucleotide in. Recent advances in bioanalysis and metabolite profiling of.
View laixin wang s business profile as vice president of bioanalytical at novabioassays llc. Many bioanalytical methods for oligonucleotides have been developed to. Quantitative bioanalysis of oligonucleotides thermo fisher scientific. If the address matches an existing account you will receive an email with instructions to reset your password. Request pdf on jun 1, 2011, laixin wang and others published oligonucleotide bioanalysis. Laixin wang, sherry liu, qiuying zhu, scott reuschel and min meng.
Strategies for bioanalysis of an oligonucleotide class. Tandem labs, 1121 east 3900 south, building c, suite 105, salt lake city, ut 84124, usa. Advances in quantitative bioanalysis of oligonucleotide biomarkers and therapeutics article in bioanalysis 82 december 2015 with 67 reads how we measure reads. Regulated bioanalysis of oligonucleotide therapeutics and. Laixin wang, sherry liu, qiuying zhu, scott reuschel and min meng tandem labs. We believe that lcmsms represents the future highthroughput platform for the bioanalysis of oligonucleotide therapeutics.
Pdf bioanalysis of sirna and oligonucleotide therapeutics in. Bioanalysis of sirna and oligonucleotide therapeutics in biological fluids and tissues. Hydrolysis of phase ii conjugates for lcms bioanalysis of. Recent advances in bioanalysis and metabolite profiling of oligonucleotide therapeutics 2019. Sensitivity versus specificity find, read and cite all the research you need on researchgate. Request pdf on nov 1, 20, laixin wang and others published regulated bioanalysis of oligonucleotide therapeutics and biomarkers. Find contacts direct phone number, email address, work history, and more. An 18mer phosphorothioate oligonucleotide tl0901 was used as a model compound to demonstrate the assay specificity and sensitivity. Bioanalysis of sirna and oligonucleotide therapeutics in. Recent advances in bioanalysis and metabolite profiling of oligonucleotide therapeutics.
The bioanalysis of oligonucleotide therapeutics is constantly evolving, primarily driven by drug development needs and regulatory requirements. Quantitation of oligonucleotides in human plasma using q. Use of lcmsms assays for macromolecules, like oligonucleotides, is challenging due to lack of sensitivity and low analyte recovery. Technical advances and demands for highthroughput accurate quantification of oligonucleotide therapeutics and biomarkers in pharmaceutical research and clinical diagnosis have aided evolution in quantitative bioanalysis of oligonucleotides.
Strategies for bioanalysis of an oligonucleotide class macromolecule from rat plasma using liquid chromatography tandem mass spectrometry. Regulated bioanalysis of oligonucleotide therapeutics and biomarkers. The importance of the length andor structure of fluorescently labeled. Samuel wainhaus associate director of bioanalytical at alnylam pharmaceuticals nucleic acidbased therapeutics. The oligonucleotides and nucleic acid interest group meeting for asms 2017 was held on. Each method has been used for different study purposes for a period of time. Provides an overview of the current stage in oligonucleotide therapeutics.
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